Applications

DNA Cycle Sequencing
automated nanoliter scale reactions, automated mag bead cleanup

Abstract

The Parallab™ 350 is a fully automated, integrated platform that performs 96, nanoliter volume, cycle sequencing reactions in parallel. A standard DNA cycle sequencing reaction on the Parallab 350 is done in a total volume of 500 nanoliters. With its proprietary Nano-Pipetter™ the Parallab 350 aspirates small and accurate reagent volumes, mixes them, performs the thermal cycling, and completes all of the purification steps within 96 glass capillaries. The output is 96 purified samples, ready for analysis on capillary electrophoresis sequencers.

Nanoliter reaction volume, reduced use of consumables and complete automation is beneficial in a variety laboratory environments allowing them to realize cost and labor savings without compromising data quality.

Conclusion

On a technical level, the Parallab 350 has high value to laboratories for these reasons:

• Reliable cycle sequencing of plasmids and PCR products
  
• Downsized reaction volumes
• Walkaway automation
  
• Utilization of commercially available magnetic bead cleanup products
• Resulting high quality sequence data

                                  Select here for downloadable data sheet

Products

Parallab350™
Nanoliter Workstation

Nanodrop™

Hamilton Thorne (only in Europe)

Email us now at:
• office@parallabs.com
• technical@parallabs.com