Applications

Molecular Diagnostics
miniaturized single base extension (SBE) diagnostic methodology

Abstract

Early detection of colorectal cancers (CRC) and neoplasias are critical for long-term patient survival. Here we present a study using stool DNA for CRC screening comparing the efficacy of our modified single base extension (SBE) diagnostic platform with that of a miniaturized SBE methodology adapted for the Parallabs 350™ nanoliter liquid handler.

Approach

• Parallab 350 system:
      - 96 capillary array (Nanopipetter™)
      - Built-in features for PCR and sequencing
  
• Miniaturize & automate PCR reactions
      - Requires concentration of DNA (maintain maximum copies/PCR)
      - Reduce Taq usage with PCR volume (5x reduction)
  
• Miniaturize & automate steps in SBE
      - Reduce to 500-1000nl reactions (10x reduction in volume & reagents)
      - System can pipette, mix, & separate magnetic beads

  Select here for downloadable report

Products

Parallab350™
Nanoliter Workstation

Nanodrop™

Hamilton Thorne (only in Europe)

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