DOPlify™ provides cell ploidy status of single cells and simultaneously cell mitochondrial DNA load using a range of different NGS platforms and workflows.

DOPlify™ captures RHS’ exclusive know-how for the lysis and whole genome amplification of single or small numbers of cells providing a solution for multiplying the limited DNA in a single cell for a range of downstream applications.

DOPlify™ is for research use only and should not be used as a clinical diagnostic.

DOPlify™ is a whole genome amplification kit designed to PCR amplify the genome from single cells and limited DNA templates.

DOPlify is unique in that it uses advanced DOP-PCR, which;

  • uses the high processivity and fidelity of new generation polymerases that are NGS application grade
  • uses ready-mixes to minimise the pipetting steps and requiring only two sample tube openings to reduce the risk of contamination and
  • provides an amplification system that is flexible enough to concurrently whole genome amplify a sample whilst specifically amplifying genes of clinical interest, reducing the risk of allele drop-out.

DOPlify™ has been validated for detecting chromosome aneuploidy using RHS’ microarray EmbryoCellect

The DOPlify™ kit contents

Each DOPlify™ kit contains reagents for amplifying 50 samples:

  • Cell lysis buffer and enzyme for lysing the sample cells
  • Whole genome amplification (WGA) reagents

The DOPlify™ Whole Genome Amplification workflow

Protocol Step Explanation
Cell lysis A gentle but effective enzyme-based lysis procedure ensures robust cell lysis and a readily accessible DNA template for whole genome amplification – 15 mins
Whole genome amplification Whole genome amplification is performed using RHS’ advanced DOP-PCR, which has been optimised for limited DNA input. DOP-PCR uses degenerate primers to initiate DNA amplification, binding across a broad range of different sequences scattered genome wide -2 ½ hours
Agarose gel assessment Following amplification, the use of agarose gel electrophoresis is recommended to ensure that cell amplification has been successful – 30 mins

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Parallabs will be attending ESHRE, alongside our partners in Barcelona 1-4 July. Vitrolife EmbryoScope Symposium You are invited to join the Vitrolife Symposium, co-organised by Japan A-PART, at the ESHRE conference. Wednesday 4 July 2018, 12:00-13:15 Forum Auditorium, Centre de Convencions Internacional de Barcelona (CIBB) Learn more about the topics here  >> Visit Vitrolife at […]

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