EmbryoCellect™ Microarray

EmbryoCellect™ uses microarray technology (array Comparative Genomic Hybridisation or aCGH) to compare the number of chromosomes in a sample cell to a known reference sample. The samples are labelled with different fluorescent dyes and the amount of fluorescence is measured and compared for each chromosome.

This provides a reliable and straightforward way to count the number of chromosomes in the test cell and detect whole chromosome aneuploidy.

EmbryoCellect™ is for research use only and should not be used as a clinical diagnostic.

Download The EmbryoCellect General Information

Download The EmbryoCellect Technical Information

 

Why EmbryoCellect™?

 

Samples can be processed in batches as small as four, which makes the workflow more flexible.

The EmbryoCellect™ kit contents

Each EmbryoCellect™ kit contains reagents for analysing 20 test samples:

  • Cell lysis buffer and enzyme for lysing the sample cells
  • Reference male gDNA
  • Whole Genome Amplification (WGA) reagents
  • Fluorescent labelling PCR reagents
  • Five patented EmbryoCellect™ microarray slides with four microarrays per slide
  • Software: A Simple Excel macro that uses a microarray scanner-generated data file

The EmbryoCellect™ Microarray workflow

Protocol Step Explanation
Cell lysis Following biopsy, a gentle but effective enzyme-based lysis procedure ensures robust cell lysis and a readily accessible DNA template for whole genome amplification – 15 mins
Whole genome amplification Whole genome amplification is performed using RHS’ DOP-PCR, which has been optimised for the RHS microarray. DOP-PCR uses degenerate primers to initiate DNA amplification, binding across a broad range of different sequences scattered genome wide -2 ½ hours
Agarose gel assessment Following amplification, the use of agarose gel electrophoresis is recommended to ensure that cell amplification has been successful – 30 mins
Labelling PCR Successfully amplified samples are fluorescently labelled by a second DOP-PCR. The test is labelled with a Cy3 equivalent dye and the reference with a Cy5 equivalent dye – 45 mins
Clean-up and nanodrop Agarose gel assessment Once purified, these labelled amplicons are again assessed using agarose gel electrophoresis and spectrophotometry to ensure adequate amplification and dye incorporation has occurred – 1 hour
Hybridisation

Microarray washing, Microarray scanning and analysis

Samples are competitively hybridized to the RHS microarray – 3 hours, to overnight

After incubation, the microarray is washed and scanned (1 hour). The ratio of test to reference dye intensity after normalization is determined using RHS proprietary analysis method, providing the ploidy status of each chromosome in each sample.

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