Why Choose VIADENT II?
- Only VIADENT II uses the same MOTILE sample for counts, motility, and viability. Using other viability options, two separate samples must be analyzed for motility, counts, and viability.
- VIADENT II only uses one stain, and staining is complete within 2 minutes. Other methods require 2 to 3 separate stains, and much longer staining times.
- VIADENT II is compatible with clear, milk-based, and egg-yolk extenders. However, use of phosphate-buffered solutions may interfere with other viability stains, such as SYBR 14.
- Low cost per test. Each VIADENT stain tube provides enough stain for 5 samples, and may be refrigerated after reconstitution for up to 3 months.
How it Works
The VIADENT stain quickly penetrates non-viable sperm heads. By comparing the cells visible under standard and fluorescent illumination, the analyzer easily determines the live cells versus the dead cells. The sperm cells are first identified and tracked under visible illumination as with standard analysis; then, fluorescence illumination is applied for one additional frame to identify non-viable sperm.
- The ‘visible’ Zoom cell screen shows the captured sperm under Visible illumination.
- The ‘fluorescent’ Zoom screen (last frame of the series) shows the non-viable sperm under fluorescence illumination.
Easy Sample Preparation
Simply stain an aliquot of the sample with the pre-packaged VIADENT stain for 2 minutes, load into the disposable analysis chamber, and place on the IVOS II stage. You are now ready to begin analysis!
The Analysis Process
- Select the VIADENT Setup and confirm illumination
- Select the analysis fields either automatically or manually.
- Press Start Scan. Using standard visible illumination, the system analyzes count, concentration, motility, and velocity under visible illumination and viability under fluorescence illumination.
Validation of an objective approach for simultaneous assessment of viability and motility of fresh and cooled equine spermatozoa.
M.T. Wessel, G.C. Althouse
Proceedings of the International Symposium on Equine Reproduction, Animal Reproduction Science 94 (2006) 21-22
“Our results suggest that the “Viadent” option of the IVOS system is capable of a rapid, accurate and objective evaluation of both viability and motility parameters using large numbers of spermatozoa. Application of this technique in the industry may prove useful in the clinical assessment of fertilizing potential of equine spermatozoa.”