Sperm Gradient Media

GM501 Gradient Media

GM501 Gradient from Gynemed is a silane-silica based density gradient medium used in ART for separation and purification of highly motile human spermatozoa.  GM501 Gradient 100%, 90% and 45% can be used in combination with IUI, IVF and ICSI.

Composition

  • GM501 Gradient 100 % is an isotonic gradient for semen preparation with a density of approximately 1.12 g/ml.
  • GM501 Gradient 90% and 45% consists of silane-coated colloidal silica particles suspended in HEPES-buffered medium

Product specifications and quality control

  • All raw materials are of highest available purity (European Pharmacopoeia and/or USP standard) if applicable.
  • A certificate of analysis is available for each batch upon request from our website with respective lot number.
  • GM501 Gradient is manufactured according to the following specifications:
pH 7.20-7.90 (Release criteria: 7.20-7.60)
Osmolality (mOsm/kg) 300-330 (GM501 Gradient 100%)
330-350 (GM501 Gradient 90%)
310-340 (GM501 Gradient 45%)
Density (g/ml) 1.1150-1.1250 (GM501 Gradient 100%)
1.1050-1.1150 (GM501 Gradient 90%)
Viscosity (cP) < 1.75
Sterility sterile – SAL10-3 (Sterility Assurance Level)
Endotoxins (EU/ml) < 0.5
Sperm Survival test (%) ≥ 80 survival after 45 minutes exposure of density selected spermatozoa to the test medium
Mouse Embryo Assay (MEA) not MEA tested

Ordering Information

Product description Product code Unit
GM501 Gradient 100 % 4 GM 501G-100-10 10 ml
GM501 Gradient 100% 4 GM 501G-100-50 50 ml
GM501 Gradient 100% 4 GM 501G-100-100 100 ml
GM501 Gradient 100% 4 GM 501G-100-250 250 ml
GM501 Gradient 90 % 4 GM 501G-90-10 10 ml
GM501 Gradient 90 % 4 GM 501G-90-50 50 ml
GM501 Gradient 90 % 4 GM 501G-90-100 100 ml
GM501 Gradient 90 % 4 GM 501G-90-250 250 ml
GM501 Gradient 45 % 4 GM 501G-45-10 10 ml
GM501 Gradient 45 % 4 GM 501G-45-50 50 ml
GM501 Gradient 45 % 4 GM 501G-45-100 100 ml
GM501 Gradient 45 % 4 GM 501G-45-250 250 ml

 

Comments are closed.