EmbryoScope

EmbryoScope-webTIME-LAPSE INCUBATOR

EmbryoScope time-lapse incubator offers improved embryo selection through undisturbed stable incubation and time-lapse monitoring.  It has been adopted worldwide and has been used to treat more than 300.000 patients.

The heart of the system is the EmbryoScope time-lapse incubator which provides a stable incubation environment while high quality images of embryo development are acquired. These images are used to create time-lapse videos of embryo development for analysis using the EmbryoViewer software.

Undisturbed stable incubation

The EmbryoScope incubator provides an extremely stable culture environment for embryos. Temperature is tightly regulated by direct heat contact and secondary temperature control in the incubation chamber and surrounding space. Air is continuously purified through a HEPA/VOC filter to ensure embryos are not exposed to any contaminants. The incubator features an integrated gas mixer for flexibility in selecting gas mixes and allows for easy and economical implementation of reduced oxygen culture. Finally, the small incubation chamber allows rapid recovery of conditions after closing the loading chamber. All instrument running conditions are registered and stored for easy review and for Quality Assurance purposes.

Optimal culture conditions:

  • Tri-gas incubator allows fast and accurate regulation of CO2 and O2 concentrations with minimal gas consumption
  • Unique temperature control by direct heat transfer to individual media-filled wells. Temperature is virtually unchanged by opening chamber (<0.2° C) when adding or removing patient samples
  • Recovery of CO2 concentration in less than 5 minutes and O2 in less than 15 minutes after closing chamber
  • Continuous circulation and purification of air supply with residence time of less than 20 minutes
  • Air purified by active carbon and HEPA filter. Removes VOCs and retains 99.97 % of particles larger than 0.3 μm
  • Simplifies compliance with EU Directive 2004/23/EC by automatic logging of running conditions such as temperature, CO2 and O2 concentration to patient data files
  • Dry incubation without water pans eliminates problems with water condensation and fungal growth on surfaces in high humidity

Time-lapse monitoring:

  • Fully automated detection and focusing of up to 72 embryos (6 patient culture dishes with 12 embryos in each dish)
  • Image acquisition in multiple focal planes of all embryos
  • High-quality Hoffman modulation contrast optics allows observation of key morphological features
  • Special Leica optics designed for red light at 635 nm to eliminate high energy light exposure

View on the online EmbryoScope Brochure

EmbryoScope Specifications

Purpose For embryo evaluation
Description Tri-gas benchtop incubator with integrated camera
Application The EmbryoScope time-lapse incubator provides an environment with controlled temperature and CO2 for the development of embryos at or near body temperature while high quality images of embryo development are acquired.

Capacity: Six disposable EmbryoSlide® culture dishes holding 12 embryos each
Dimensions: 60.3 x 56.0 x 43.5) cm / 23.7 x 22 x 17.2 in
Weight: 60 kg / 121 lbs
Input voltage: 110-240 V AC
Power consumption: 120 W
Operating range: 20 °C – 30 °C

Image acquisition: Select up to 17 focal points at each time point in user defined vertical increments
Built-in microscope: Leica 20x, 0.40 LWD Hoffman modulation contrast objective specialised for 635 nm illumination
Camera resolution: 1280 × 1024 pixels, 3 pixels per μm, monochrome, 8-bit
Embryo illumination: ≤ 0.032s per image using single red LED (635nm) gives 34μW cm-2 for image acquisition
Time between acquisitions: 10 min. cycle time for 7 focal planes, 2 min cycle time with single focal plane

Tri-gas incubator integrated in instrument
Temperature: 30 °C to 45 °C ± 0.2 °C
Oxygen: 5 % to 20 % ± 0.3 %
CO2: 2 % to 10 % ± 0.2 %
Active air circulation: Full purification of gas volume every 20 minutes
Volatile organic compounds: Removed by active carbon filter
Particles: Removed by HEPA filter which retains 99.97 % particles >0.3 μm

Europe: CE-marked class IIa medical device
USA: FDA 510(k) clearance

 

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